2D Proteome Analysis Protocols (Methods in Molecular Biology, 112),Used
2D Proteome Analysis Protocols (Methods in Molecular Biology, 112),Used

2D Proteome Analysis Protocols (Methods in Molecular Biology, 112),Used

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With the completion of sequencing projects and the advancement of a lytical tools for protein identification, proteomicsthe study of the expressed part of the genomehas become a major region of the burgeoning field of functional genomics. Highresolution 2D gels can reveal virtually all p teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2D protein gels. 2D Proteome Analysis Protocols covers all aspects of the use of 2D protein electrophoresis for the analysis of biological problems. The contri tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of highresolution 2D gels, immobilized pH gradients, computer analysis, and mass spectromet based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistryespecially in the fields of molecular biology, biochemistry, microbiology, and cell biologyshould find this book eminently useful. 2D Proteome Analysis Protocols takes the researcher through the c plete process of working with 2D protein gels from making the protein tract to finally identifying the proteins of interest. It includes protocols for generating 2D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

⚠️ WARNING (California Proposition 65):

This product may contain chemicals known to the State of California to cause cancer, birth defects, or other reproductive harm.

For more information, please visit www.P65Warnings.ca.gov.

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