Title
2'Desoxyisoguanosin: Synthese, Desoxygenierung und Einbau in Oligonucleotide (German Edition),Used
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Diplomarbeit aus dem Jahr 1993 im Fachbereich Chemie Organische Chemie, Note: 1,5, Universitt Osnabrck, Sprache: Deutsch, Abstract: 1) 2'Desoxyisoguanosin (1) wurde in einer zweistufigen Synthese dargestellt. Zu diesem Zweck wurde 2'Desoxyguanosin (3) zunchst in 2Amino2'desoxyadenosin (5) berfhrt. Dieses wurde in 2'Desoxyisoguanosin (1) umgewandelt. Bei der Reaktion von 2'Desoxyisoguanosin (1) mit Chloracetaldehyd bildete sich N6Etheno2'desoxyisoguanosin (9). Dieses zeigte mit N6Ethenoadenosin (6) vergleichbare Fluoreszenzeigenschaften. 2) 2',3'Didesoxyisoguanosin (2) konnte ber den photochemischen Austausch des 2Substituenten von 2Chlor2',3'didesoxyadenosin (20) dargestellt werden. Verbindung 20 konnte ber zwei Synthesewege gewonnen werden: Anfnglich ausgehend von 2Chlor2'desoxyadenosin (16) ber BartonDesoxygenierung in 27 % Gesamtaubeute ber vier Stufen. Alternativ wurde in einem konvergenten Syntheseweg der 2',3'Didesoxyzucker 21 mit 2,6Dichlorpurin (23) unter MitsunobuBedingungen umgesetzt. Durch nachfolgenden Austausch des 6Substituenten gegen NH2 waren die anomeren 6Amino2chlor2',3'didesoxynucleoside (25a, 25b) zugnglich. 3) Mit Hilfe der Festphasensynthese wurde das Oligomer d(iGC)3 (32) synthetisiert und seine Zusammensetzung durch enzymatische Hydrolyse bewiesen. Anhand des TmWertes von 29 C konnte die iGddCBasenpaarung nachgewiesen werden. Da alternierende iGddC Sequenzen nur unter paralleler Strangorientierung selbstkomplementr sind, resultiert im Vergleich zu d(GC)3 (33) der Verlust eines Basenpaares. Der parallele Duplex von d(iGC)3 wies mit 29C einen geringeren TmWert als d(GC)3 (45C) auf. Mit Hilfe von Inkrementberechnungen fr die freie Enthalpie pro Basenpaar, konnte bewiesen werden, da diese Destabilisierung von d(iGC)3 (32) durch die Einbue eines Basenpaares ausgelst wurde.
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Shipping & Returns
Shipping
We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
Returns
To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.
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Warranty
We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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