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Aqueous TwoPhase Systems: Methods and Protocols (Methods in Biotechnology, 11),New
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A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn ogy for product separations. Separation of biological molecules and particles in these aqueous twophase systems (ATPS) was initiated over 40 years ago by P.. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for largescale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of twophase systems has for a long time been confined to selected labora ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ twophase partitioning techniques in both basic and applied research.
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