cDNA Library Protocols (Methods in Molecular Biology, 69),Used

cDNA Library Protocols (Methods in Molecular Biology, 69),Used

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The first libraries of complementary DNA (cDNA) clones were con structed in the midtolate 1970s using RNAdependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into doublestranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con structing and screening cDNA libraries. It is not the aim of cDNA Library Protocols to give a comprehensive review of all cDNA librarybased methodologies; instead we present a series of uptodate protocols that together should give a good grounding of proce dures associated with the construction and use of cDNA libraries. In deciding what to include, we endeavored to combine uptodate versions of some of the most widely used protocols with some very usefiil newer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 15 concentrate on cDNA library construction and manipula tion, Chapters 6 and 7 describe means of cloning difficulttoobtain ends of cDNAs, Chapters 818 give various approaches to the screening of cDNA libraries, and the remaining chapters present methods of analysis of cDNA clones including details of how to analyze cDNA sequence data and how to make use of the wealth of cDNA data emerging from the human genome project.

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