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CellBased Microarrays: Methods and Protocols (Methods in Molecular Biology, Vol. 706) (Methods in Molecular Biology, 706),Used
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As a high throughput method for analyzing gene function, cellbased microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In CellBased Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cellbased microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for overexpression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cellbased array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cuttingedge, CellBased Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
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