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Of the handful of neurotransmitters modulating taste response, norepinephrine (NE) may be the least understood. Although the physiological role of NE has been tested, it is still unclear whether the rat taste bud may serve as an endogenous source of neurotransmitter or if it must be taken up from outside the taste bud. Immunocytochemical analysis of DBH has shown that DBHLIR is present in taste cells of the rat circumvallate papillae. Specifically, DBHLIR is present in a subset of Type II taste cells. Nearly all (98%) DBHLIR cells express PLC2LIR, and about half (41%) of PLC2LIR cells express DBHLIR. DBHLIR does not colocalize with NCAM, and thus, is not present in Type III cells. NETLIR is present in the taste cells of the rat circumvallate papillae. Double labeling of NETLIR and IP3R3LIR indicates that NET is present in Type II taste cells. A large proportion (77%) of NETLIR cells express IP3R3LIR and all (100%) IP3R3LIR cells express NETLIR. A small proportion (7.8%) of NETLIR cells also express syntaxin1LIR in Type III cells. About a third (32.8%) of syntaxin1LIR cells express NETLIR.
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