Development of N gene mediated resistance in tomato against GBNV: Development of nucleocapsid gene mediated resistance in tomato,Used

Development of N gene mediated resistance in tomato against GBNV: Development of nucleocapsid gene mediated resistance in tomato,Used

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Bud blight is an emerging viral problem in tomato. Bud blight affected plants reacted positively to polyclonal antiserum of Groundnut Bud necrosis Virus (N gene). TNCo, UPKa, MHPu and MHRa isolates could infect 13 out of 27 plant species tested belonging to Solanaceae, Leguminoase and Malvaceae. The nucleocapsid protein (N) and movement protein (NSm) genes were cloned and sequenced using GBNV specific primers. N gene of the tomato Tospovirus isolates shared 97100% amino acid sequence identity with GBNV (type isolate). Further the N gene among GBNV isolates originating from tomato and other hosts and different locations was highly conserved (9699%), suggesting their common origin. Similarly the NSm genes of tomato Tospovirus isolates from different locations shared 98% amino acid sequence identity with GBNV (type isolate) and were highly conserved (9698%). N gene from GBNV TNCo isolate was used as transgene to confer resistance in tomato. Nine transgenic lines Co3S1, Co3S2, Co3S12, PRS1, PRS3, PRA1, PRA2, PRA4 and PRA5 were Southern positive. None of the T0 lines showed symptoms upon mechanical inoculation with TNCo isolate

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Shipping
We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.

Returns
To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.

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Warranty

We provide a 2-year limited warranty, from the date of purchase for all our products.

If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.

This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).

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