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This book presents not only the simultaneous combination of optical methods based on holographic principles for markerfree imaging, realtime trapping, identification and tracking of micro objects, but also the application of substantial low coherent light sources and nondiffractive beams. It first provides an overview of digital holographic microscopy (DHM) and holographic optical tweezers as well as nondiffracting beam types for minimalinvasive, realtime and markerfree imaging as well as manipulation of micro and nano objects.It then investigates the design concepts for the optical layout of holographic optical tweezers (HOTs) and their optimization using optical simulations and experimental methods. In a further part, the book characterizes the corresponding system modules that allow the addition of HOTs to commercial microscopes with regard to stability and diffraction efficiency. Further, based on experiments and microfluidic applications, it demonstrates the functionality of the combined setup, and discusses several types of nondiffracting beams and their application in optical manipulation. The book shows that holographic optical tweezers, including several nondiffracting beam types like Mathieu beams, combined parabolic and Airy beams, not only open up the possibility of generating efficient multiple dynamic traps for micro and nano particles with forces in the pico and nano newton range, but also the opportunity to exert optical torque with special beams like Bessel beams, which can facilitate the movement and rotation of particles by generating microfluidic flows. The last part discusses the potential use of a slightly modified DHMHOTsystem to explore the functionality of direct laser writing based on a two photon absorption process in a negative photoresist with a continuous wave laser
⚠️ WARNING (California Proposition 65):
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birth defects, or other reproductive harm.
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