Does the cytoskeleton manipulate the auxin: induced changes in the endoplasmic reticulum,Used

Does the cytoskeleton manipulate the auxin: induced changes in the endoplasmic reticulum,Used

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SKU: DADAX383839304X
Brand: LAP Lambert Academic Publishing
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The variation in ER structure and motility under different stages of cell development remain largely unexplored. Here, the ER structure is observed along with the changes that take place within this structure over time in growing and nongrowing live epidermal cells of the pea tendril. The ER was labeled by green fluorescent protein, fused to the KDELER. Retention signal and confocal laser scanning microscopy was used to localize the fluorescent signal. It was found that both the structure and motility of growing cells was different from the nongrowing cells. The growing cells had a more open arrangement of the cortical ER, fewer lamellae and showed greater tubular dynamics, while the nongrowing cells had a denser arrangement of the cortical ER network, with more lamellae and less tubular dynamics. Furthermore, it was found that these observed differences in the cortical ER structure and dynamics were due to growth. Similar observations were made in nongrowing cells induced to grow by the exogenous application of auxin. These changes in the ER were dependent on both microtubules and actin cytoskeleton networks.

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