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Electron Microscopy Methods and Protocols (Methods in Molecular Biology),Used
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Handson experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological Xray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and proteinnucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemicallyfixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a goldstandard laboratory manual of cuttingedge electron microscopy techniqueseach optimized for reproducibility and robust resultstoday's goldstandard laboratory manual.
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