Title
Elimination of a selectable marker gene in barley: via segregation of uncoupled TDNAs in populations of doubled haploids,Used
Delivery time: 8-12 business days (International)
Barley represents one of the economically most important and widely distributed crops worldwide, and genetic engineering is expected to play a crucial role in its further improvement. However, once transgenic plants are obtained, the selectable marker gene is not necessary anymore or even unwanted, and thus viable means to circumvent the use of selectable markers or to remove them from transgenic plants are required. In the present study, a novel strategy has been pursued to generate selectable markerfree transgenic lines instantly homozygous for the geneofinterest. Primary cotransgenic plants (T0) containing both the selectable marker gene (hygromycin phosphotransferase) and a model geneofinterest (glucuronidase) were produced via Agrobacteriummediated gene transfer using two independent TDNAs. Both removal of the selectable marker and establishment of instant homozygosity of the geneofinterest was then achieved at a time through segregation of uncoupled TDNAs in populations of doubled haploid (DH) lines produced from the T0 plants via embryogenic pollen culture.
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⚠️ WARNING (California Proposition 65):
This product may contain chemicals known to the State of California to cause cancer, birth defects, or other reproductive harm.
For more information, please visit www.P65Warnings.ca.gov.
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Shipping & Returns
Shipping
We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
Returns
To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.
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Warranty
We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
We accept payments with :
Visa, MasterCard, American Express, Paypal, Shopify Payments, Shop Pay and more.