Title
Evaluation ATPase activity of purified proteins: Expression, purification and evaluation ATPase activity of essential tellurite ,Used
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Tellurite resistance (TeR) determinat from uropathogenic strain Escherichia coli KL53 is harbored on conjugative plasmid pTE53, which consists of two clusters of ter genes. The first cluster of genes is responsible for the TeR terZABCDEF and protective region consists of terXYW. Only four genes terBCDE are essential for TeR and these genes were object of our study. The expression, purification and evaluation ATPase activity of the tellurite resistance operon is a part of our work. We have identified that Cterminus of these proteins is crucial for maintain of their function due to loss of original function after modification by Histag. We established that protein TerC is membrane situated with 9 transmembrane domains and TerBDE are soluble cytosolic proteins.We have identified the dimer formation of TerB, TerD and TerE and interactions between TerD and TerE. The interaction between TerB and TerD/TerE were also detected. In the last part of this study we examined the response to oxidativ stress of TeR strains and strains harboring different part of TeR operon. We have also measured the total anitioxidant capacity of selected strains.
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We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
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We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
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