Title
Evaluation of Sigatoka leaf spot (Mycosphaerella musicola Leach): disease resistance in banana using morphological and molecular,Used
Delivery time: 8-12 business days (International)
Sigatoka leaf spot disease limits banana production. Breeding for resistance is the most effective method to control Musa diseases. However, Musa improvement using conventional methods is difficult due to lack of genetic variability, hence biotechnology. Markerassisted selection is a reliable method to improve disease resistance in Musa. This study was to identify markers that may be linked to Sigatoka leaf spot disease in Musa, using RAPDs and converting such into SCAR. A total of 102 oligonucleotide OPERON primers were used to screen genomic DNA from resistant and susceptible cultivars with only 10.8% polymorphism. Eventually, OPK01 and OPK11 primers in Calcutta 4 were eluted, but only OPK 11 was sequenced and cloned using pGEM2T vector, resulting to a band size of 4.3 KB, and the development of two SCAR markers. A FASTA search in the Musa database could not identify homologous gene sequences to the PCR fragment. The SCAR marker was used to amplify genomic DNA from the segregating population which could not discriminate between resistant and susceptible samples due to amplification conditions, limited number of primers and absence of tight linkage with the gene of interest.
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⚠️ WARNING (California Proposition 65):
This product may contain chemicals known to the State of California to cause cancer, birth defects, or other reproductive harm.
For more information, please visit www.P65Warnings.ca.gov.
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Shipping & Returns
Shipping
We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
Returns
To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.
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Warranty
We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
We accept payments with :
Visa, MasterCard, American Express, Paypal, Shopify Payments, Shop Pay and more.