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This research work was performed to examine the feasibility of using Fourier transform infrared (FTIR) spectroscopy (in the midIR range, 4000400 cm1) to i) detect and identify bacterial cells. The greatest spectral variation was observed between 1800 cm1 and 900 cm1: for amide I band at ~1650 cm1, amide II band at ~1540 cm1, CH3 and CH2 of protein components around 1455 cm1 and 1398 cm1, P=O of the phosphodiester backbone of nucleic acids around 1242 cm1 and 1080 cm1, and polysaccharide compounds around 1050 950 cm1. From the second derivative transformation and loadings plot, FTIR showed the potential to detect sublethally injured and dead cells and discriminate between different types and degrees of cell injury.
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