Functional Genomic Analysis of Pseudorabies Virus: Functional Analysis of Pseudorabies Virus Genes by RealTime RT PCR and Knock,Used

Functional Genomic Analysis of Pseudorabies Virus: Functional Analysis of Pseudorabies Virus Genes by RealTime RT PCR and Knock,Used

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SKU: DADAX3843389489
Brand: LAP Lambert Academic Publishing
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The pseudorabies virus (PRV) is a neurotropic herpesvirus, it is a pathogen of swine, causing Aujeszky's disease. PRV is a widely used model organism for studies in pathogenesis and molecular biology. This virus is also utilized as a neural circuit tracer. We have developed (1) a realtime RTPCR method for the global analysis of PRV gene expression; (2) a novel calculation method to examine the relative expression ratios, and which is applicable for the analysis of gene expression in any genetic system that progressively changes in time; (3) PRVbased troponeon expressing transsynaptic tracers which label synaptically connected nerve cells; (4) transgenic viruses encoding differentially colored fluorescent proteins (FPs) and multiple colored FPs to differentiate the brain regions; (5) a method for a shortterm culture of isolated cardiac muscle cells that retain their morphological and physiological integrity; (6) a PRVbased system for delivery of foreign genes to cardiomyocytes.

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