Title
G Protein Methods and Protocols: Role of G Proteins in Psychiatric and Neurological Disorders (Neuromethods, 31),Used
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The G proteins are a family of structurally homologous, plasma membraneassociated guaninenucleotidebinding proteins. These proteins play an integral role in the tra duction of extracellular signals through second messenger systems. As such, G proteins affect a wide variety of intra cellular biochemical reactions by regulating the concent tion of second messengers in cells. G proteins are heterotrimeric, consisting of a, p, and y polypeptide chains, with G protein specificity largely det mined by the asubunit, Molecular cloning of G protein s units has revealed 23 distinct asubunits, encoded by 17 different genes. Based on functional measures, G proteins are generally classified into three major categories: the G, family, which is stimulatory for adenylyl cyclase; the G, f ily, which is inhibitory for adenylyl cyclase; and the G, f ily, which stimulates phospholipases (Birnbaumer and Birnbaumer, 1995). Alternatively, on the basis of sequence homology, G proteins can be subdivided into four cate ries: G,, G,, G,, and G12.
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