Title
Gene Targeting: A Practical Approach (The ^APractical Approach Series),Used
Delivery time: 8-12 business days (International)
The book begins by describing in detail the rationale behind designing gene targeting vectors, including both basic replacement vectors for creating null mutations and more sophisticated twostep approaches for creating subtle mutations. It describes the latest techniques for culturing andmanipulating ES cells and a number of approaches for identifying rare targeted cell clones. Techniques for culturing and assaying for mouse and human hematopoietic progenitors in vitro and in vivo are then covered, followed by the design of, and use of, retrovirus vectors for introducing andexpressing genes in bone marrow cells. Two approaches for making ES cell chimeras are described. One chapter covers the more standard approach of ES cell injection into blastocysts as well as morula injection. Another chapter describes techniques for making aggregation chimeras and in particulara recent approach using tetraploid host embryos to produce completely ES cellderived embryos and mice. The critical steps for obtaining germline chimeras are discussed. The book is concluded by a discussion of the many uses of enhancer, gene and promoter trap vectors, the advantages of each, anddescribes in detail the techniques for producing and analyzing "trap" vector insertions in ES cells. The protocols are written with sufficient detail for a novice in the area to use them as a manual to learn the various techniques required for producing mutant mice, beginning with the embryonicstem cells and ending up with chimeric mice for breeding.
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⚠️ WARNING (California Proposition 65):
This product may contain chemicals known to the State of California to cause cancer, birth defects, or other reproductive harm.
For more information, please visit www.P65Warnings.ca.gov.
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Shipping & Returns
Shipping
We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
Returns
To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.
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Warranty
We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
We accept payments with :
Visa, MasterCard, American Express, Paypal, Shopify Payments, Shop Pay and more.