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Understanding the mechanisms and control of gene transcription is one of the central goals for a large proportion of molecular biologists currently involved in research. This book concentrates on RNA polymerase II transcription of eukaryotic proteincoding systems but many of the approaches and techniques described apply equally well to the study of systems involving RNA polymerases I and III. This book covers all the major current procedures and approaches in this field, including not only the analysis of transcription in vitro and in vivo but also the identification, purification and characterization of transcription factors and their interaction with specific DNA target sites. Appendices list useful reference data including details of all known transcriptional control ciselements and transfactors for RNA polymerase II genes, complete with a full bibliography.
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