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This book presents a descriptive, comprehensive catalogue of viral and cellular cisacting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. Included is a selection of optimized, stateoftheart protocols for transferring genes into mammalian cells and subsequently assaying for transfer and expression of the genes of interest. This unique volume describes in detail essential techniques for propagating cells and cell lines, DNA transfer techniques, drug selection and gene amplification techniques, expression cloning, subtractive hybridization and retrovirusmediated gene transfer techniques. Also featured are highly sensitive assays for gene transfer and expression, including detection of DNA and RNA by both isotopic and nonisotopic detection methods as well as the most sensitive nonisotopic Western techniques for the detection of proteins.
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birth defects, or other reproductive harm.
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