Title
Hemmung der Expression von porzinen endogenen Retroviren (PERVs) mittels RNAInterferenz (German Edition),Used
Delivery time: 8-12 business days (International)
Diplomarbeit aus dem Jahr 2009 im Fachbereich Biologie Mikrobiologie, Molekularbiologie, Note: 1,3, Universitt Potsdam (Robert KochInstitut), Sprache: Deutsch, Abstract: Die Xenotransplantation vom Schwein auf den Menschen ist mit dem Risiko der bertragung von porzinen endogenen Retroviren (PERVs) verbunden, die im Genom jeder Zelle des Schweins integriert sind und sich nicht durch DPF(designated pathogen free) Zucht eliminieren lassen. PERVs infizieren humane Zellen in vitro, womit die Mglichkeit einer in vivo Infektion des humanen immunsuppremierten Rezipienten nicht ausgeschlossen werden kann. In den letzten Jahren wurde deshalb nach Mglichkeiten gesucht, die bertragung zu verhindern. Die RNAInterferenz stellt hierbei eine vielversprechende Strategie dar, da sie die Replikation von PERV posttranskriptional unterbinden kann. So gelang es, transgene Schweine zu generieren, die in der Lage waren, shRNAs stabil zu exprimieren. In isolierten primren Fibroblasten der transgenen Schweine wurde das fluoreszierende GFP mittels Fluoreszenzmikroskopie sowie die Integration des Transgens mittels PCR nachgewiesen, indem zum einen das gfpGen und der verwendete lentivirale Vektor, als auch die Expressionskassette der shRNA detektiert wurden. Alle entnommenen primren Fibroblasten enthielten Proviren der Subtypen A, B und C, die mittels PCR nachgewiesen wurden. Zudem konnte die Expression der VolllngenmRNA sowie gespleiter envmRNA mittels RTPCR gezeigt werden. Um die Sensitivitt der Nachweismethoden der PERVExpression zu erhhen, wurde eine neue onestep RT realtime PCR unter Verwendung neuer Primer und Sonde etabliert. Dadurch konnte eine verbesserte Detektion der shRNAinduzierten Hemmung der PERVExpression um bis zu 93 % anstelle frherer 70 % nachgewiesen werden. Um die Effektivitt der RNAInterferenz weiter zu erhhen, wurden neue PERVmRNAspezifische shRNAs generiert und mit der bisher verwendeten shRNA zu Tandems kombiniert. Nach der Klonierung der
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Shipping & Returns
Shipping
We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
Returns
To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.
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Warranty
We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
We accept payments with :
Visa, MasterCard, American Express, Paypal, Shopify Payments, Shop Pay and more.