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Human Retrovirus Protocols: Virology and Molecular Biology (Methods in Molecular Biology, 304),New
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A cuttingedge collection of basic and stateoftheart methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cuttingedge methods for exploring the interplay between the viruses and the host. Here, the researcher will find uptodate techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV1, the coreceptor usage of HIV1, and human gene expression with HIV1 infection by microarrays, as well as for phenotyping HIV1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV1 cofactors that are critical for HIV1 infection.
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