Title
Investigation of Cytoskeletal Systems with Optical Tweezers: Adhesion Forces of Actin Filaments and Malaria Parasites Measured w,Used
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Optical tweezers are a versatile tool to apply and measure forces in the piconewton range on microscopic objects that are held by optical forces in a focused laser beam. We employed holographic optical tweezers (HOT) to create extended force sensor arrays, consisting of multiple trapped particles that were controlled and probed individually. The combination of highspeed video microscopy with fluorescence imaging allowed the visualization of labeled protein structures in parallel with the tracking of multiple trapped particles for force measurements. Using this setup, we could perform quantitative force measurements on biological samples with HOT for the first time. This provided the possibility to measure dynamic processes such as the contractility of twodimensional crosslinked actin networks in the microfluidic flow cell. To investigate bundling processes between filaments, a method has been established using dynamic HOT to manipulate zipperlike structures between trapped particles actively. In a different set of experiments, the adhesion process of the Malaria parasite Plasmodium was investigated.Using optical tweezers, the formation of adhesion sites was probed.
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We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
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We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
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