Isolation and characterization of new psychrophilic bacteria; Cloning and characterization of industrial relevant pullulanase an,Used

Isolation and characterization of new psychrophilic bacteria; Cloning and characterization of industrial relevant pullulanase an,Used

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In the present study, two psychrophilic bacteria were isolated and characterized from sea water sample collected from Spitzbergen in the Arctic. The psychrophilic strictly anaerobic Ilyobacter psychrophilus sp. nov. and the psychrophilic aerobic bacterium Shewanella arctica sp. nov.. Two industrial relevant enzymes were cloned and characterized from the new isolated bacterium Shewanella arctica sp. nov.. A type I pullulanase and a subtilisinlike serine protease. In der vorliegenden Studie wurden aus aus Spitzbergen in der Arktis stammenden Seewasserproben zwei neue, psychrophile Bakterienspezies isoliert und charakterisiert. Das strikt anaerobe Bakterium Ilyobacter psychrophilus sp. nov. und das aerobe Bakterium Shewanella arctica sp. nov. Aus Shewanella arctica sp. nov. wurden zwei industriell relevante Gene isoliert, kloniert und rekombinant exprimiert und die rekombinanten Enzyme gereinigt und charakterisiert. Bei den Enzymen handelt es sich um eine Typ I Pullulanase und eine Subtilisinhnliche Serinprotease.

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