Molecular Cloning: A Laboratory Manual, Third Edition (3 Volume Set)

Molecular Cloning: A Laboratory Manual, Third Edition (3 Volume Set)

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The First Two Editions Of This Manual Have Been Mainstays Of Molecular Biology For Nearly Twenty Years, With An Unrivalled Reputation For Reliability, Accuracy, And Clarity. In This New Edition, Authors Joseph Sambrook And David Russell Have Completely Updated The Book, Revising Every Protocol And Adding A Mass Of New Material, To Broaden Its Scope And Maintain Its Unbeatable Value For Studies In Genetics, Molecular Cell Biology, Developmental Biology, Microbiology, Neuroscience, And Immunology. Handsomely Redesigned And Presented In New Bindings Of Proven Durability, This Threevolume Work Is Essential For Everyone Using Today'S Biomolecular Techniques. The Opening Chapters Describe Essential Techniques, Some Wellestablished, Some New, That Are Used Every Day In The Best Laboratories For Isolating, Analyzing And Cloning Dna Molecules, Both Large And Small. These Are Followed By Chapters On Cdna Cloning And Exon Trapping, Amplification Of Dna, Generation And Use Of Nucleic Acid Probes, Mutagenesis, And Dna Sequencing. The Concluding Chapters Deal With Methods To Screen Expression Libraries, Express Cloned Genes In Both Prokaryotes And Eukaryotic Cells, Analyze Transcripts And Proteins, And Detect Proteinprotein Interactions. The Appendix Is A Compendium Of Reagents, Vectors, Media, Technical Suppliers, Kits, Electronic Resources And Other Essential Information. As In Earlier Editions, This Is The Only Manual That Explains How To Achieve Success In Cloning And Provides A Wealth Of Information About Why Techniques Work, How They Were First Developed, And How They Have Evolved.

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Frequently Asked Questions

  • Q: What is the main focus of 'Molecular Cloning: A Laboratory Manual, Third Edition'? A: The main focus of this manual is to provide comprehensive protocols and techniques for molecular cloning, including DNA isolation, analysis, and cloning methods used in various fields such as genetics and microbiology.
  • Q: Who are the authors of this laboratory manual? A: The authors of this laboratory manual are Joseph Sambrook and David Russell, who have updated and revised the content to reflect current practices in molecular biology.
  • Q: How many volumes does this manual contain? A: This manual is a three-volume set, offering extensive coverage of molecular cloning techniques.
  • Q: What topics are covered in 'Molecular Cloning: A Laboratory Manual'? A: The manual covers essential techniques like cDNA cloning, DNA amplification, mutagenesis, DNA sequencing, and methods for analyzing transcripts and proteins.
  • Q: Is this manual suitable for beginners in molecular biology? A: Yes, the manual is designed to be accessible for users at all levels, providing clear explanations of techniques as well as detailed protocols.
  • Q: What kind of additional resources does this manual include? A: The manual includes an appendix that compiles essential information such as reagents, vectors, media, technical suppliers, and electronic resources.
  • Q: In what condition is this book available? A: The book is available in new condition, ensuring that all content is fresh and up-to-date.
  • Q: When was the third edition of this manual published? A: The third edition was published on January 1, 2001.
  • Q: What is the page count of the entire three-volume set? A: The entire three-volume set contains a total of 2344 pages.
  • Q: What type of binding does this laboratory manual have? A: This laboratory manual is bound in paperback, offering flexibility and durability for laboratory use.