Molecular Detection and Quantification of BCRABL gene: Molecular detection,Used

Molecular Detection and Quantification of BCRABL gene: Molecular detection,Used

SKU: DADAX3659202924 In Stock
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This study was designed as a try to apply molecular, conventional cytogenetic, and immunological techniques as conformational diagnosis of bcrabl gene and protein, and also to follow up chronic myeloid leukemia patients (CML) treated with imatinb mesylate (IM) for assessment of response at different IM treatment durations. Molecular screening for the presence of bcrabl gene in 34 CML patients and negative controls were done using MultiplexSingle StepReverse TranscriptasePolymerase Chain Reaction. The results showed that all CML patients were positive for bcrabl gene while all the others were negative for this gene. For assessment of molecular response to IM, quantification of bcrabl gene in CML patients were done using Real TimeReverse TranscriptasePolymerase Chain ReactionTaqman.CML patients, who show signs of treatment nonresponse or relapse, were screened for T315I mutation using Allele Specific oligonucleotides. The results showed that this mutation dose not detected in any of those patients.In conclusion, obligatory complementary tests that should be performed for any CML patient who start to receive IM, in order to assess the full respons to therapy.

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Warranty

We provide a 2-year limited warranty, from the date of purchase for all our products.

If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.

This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).

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