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Single cell analysis has typically been difficult and challenging to perform in such a way that meaningful statistical information can be extracted. This thesis documents the development of a microchip or labonachip platform to perform single cell electrophoresis in parallel separation channels. It describes the use of optical tweezers to select a cell and transport it to a desired separation channel. It also describes the use of electromechanical shearing to lyse a cell. These two techniques are combined with lasermicromachined separation channels to perform simple electrohoresis on the fluorescently labeled cellular contents.
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This product may contain chemicals known to the State of California to cause cancer,
birth defects, or other reproductive harm.
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