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Phosphorous31 NMR: Principles and Applications focuses on the evolution of phosphorus31 nuclear magnetic resonance (NMR) as a widely applied spectroscopic probe of the structure and dynamics of phosphoruscontaining compounds. The selection first offers information on the principles and empirical observations of phosphorus31 chemical shifts and the principles and applications of phosphorus31 spinspin coupling constants. Discussions focus on introduction and basic principles, directly bonded phosphorus coupling constants, and twobond and threebond coupling constants. The text then ponders on phosphorus31 NMR of enzyme complexes and paramagnetic probes of enzyme complexes with phosphoruscontaining compounds. The manuscript elaborates on the use of chiral thiophosphates and the stereochemistry of enzymatic phosphoryl transfer and DNA and RNA conformations. Topics include helixcoil transitions, sequence dependence of doublestranded DNA conformations, dynamic behavior of RNA and DNA, biological and genetic significance, and stereochemical problems studied with chiral thiophosphates. The conformation and dynamics of nucleic acids and phosphoproteins, relaxation behavior of nucleic acids, and theory and applications of solidstate phosphorus31 NMR to nucleic acids are also discussed. The selection is a valuable reference for readers interested in the principles and applications of phosphorus31 nuclear magnetic resonance.
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