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Prokaryotic Genomics,Used
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This manual reflects practical approaches to handling bacteria in the labora tory. It is designed to recall historical methods of bacterial genetics that have had recent developments and to present new techniques that allow full genome analysis. It has been written for microbiologists who need to group their protocols at the state of the art of a new millennium and also for scientists in other fields of life sciences who need to use bacteria for their research. Teachers, graduate students, and postdocs also will benefit from having these protocols to help them understand modern bacterial genetics. I learned so much from these contributions from my colleagues that I have no doubt about the daily usefulness of this book. April 2002 Michel Blot XII Abbreviations AcylHSL Nacyl homoserine lactone moi multiplicity of infection Amp or Ap ampicillin N amino C carboxy NMR nuclear magnetic resonance CIOHSL NdecanoylLhomoserine lactone 30HC14:1HSL N(3hydroxy7 cistetra C12HSL NdodecanoylLhomoserine lac decanoyl)homoserine lactone tone 30HC4HSL N3hydroxybutanoylL C14HSL NtetradecanoylLhomoserine homoserine lactone lactone ONPG onitrophenyl Dgalactopyranoside C4HSL NbutanoylLhomoserine lactone ORF open reading frame C6HSL NhexanoylLhomoserine lactone OTG ISoctyl Dthioglucoside C8HSL NoctanoylLhomoserine lactone 3oxoCIOHSL N3oxodecanoylLhomo Cam or Cm chloramphenicol serine lactone CBD chitin binding domain 3oxoC12HSL N3oxododecanoylL CHEF contour clamped homogenous electric homoserine lactone field 3oxoC14HSL N3oxotetradecanoylL CI consistency index homoserine lactone CRIM conditionalreplication, integration, 3oxoC4HSL N3oxobutanoylLhomoser and modular ine lactone dCTP deoxycytidine triphosphate 3oxoC6HSL N3 oxohexanoylLhomoser deg.'
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