Protein Microarrays Based on Recombinant Antibody Fragments: Catcher and Carrier a Crucial Combination,Used

Protein Microarrays Based on Recombinant Antibody Fragments: Catcher and Carrier a Crucial Combination,Used

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SKU: DADAX3843375690
Brand: LAP Lambert Academic Publishing
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DNA microarray is a wellestablished technology for gene expression profiling throughout biological research and clinical diagnostics. However, the development of comparable technologies on protein level has turned out to be complicated by the distinct nature of individual protein molecules. Here, we suggest the use of structurally uniform recombinant antibody molecules as catcher molecules for protein analytes. In the scope of this thesis, the catcher molecule has been discussed in terms of sensitivity of detection, onchip stability and the format antibodymolecules can be spotted onto the solid support. At the same time, different carriers have been evaluated with regard to their structure (1, 2 or 3dimensional) and coupling chemistries (adsorption, covalent coupling or affinity coupling). Thus, this thesis provides important technological insides, of use for anyone intending to perform protein profiling in large scale and high throughput manner.

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This product may contain chemicals known to the State of California to cause cancer, birth defects, or other reproductive harm.

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