Proteomics Approaches For Studing Retrograde Transport: Analyzing The Dynamic Protein Traffic,Used

Proteomics Approaches For Studing Retrograde Transport: Analyzing The Dynamic Protein Traffic,Used

In Stock
SKU: DADAX3659172022
Brand: LAP Lambert Academic Publishing
$124.05 $177.21
Save $53.16
Quantity
Add to wishlist
Add to compare

Processing time: 1-3 days

US Orders Ships in: 3-5 days

International Orders Ships in: 8-12 days

Return Policy: 15-days return on defective items

Payment Option
Payment Methods

Help

If you have any questions, you are always welcome to contact us. We'll get back to you as soon as possible, withing 24 hours on weekdays.

Customer service

All questions about your order, return and delivery must be sent to our customer service team by e-mail at yourstore@yourdomain.com

Sale & Press

If you are interested in selling our products, need more information about our brand or wish to make a collaboration, please contact us at press@yourdomain.com

Proteomics is a powerful technique for the largescale identification of proteins. A number of proteomics approaches have been developed to study protein localization at steady state. Here we report a novel dynamic proteomics strategy to identify plasma membrane proteins that undergo retrograde transport to the transGolgi network. This strategy is based on the covalent modification of the plasma membrane proteome with a benzylguanine derivative. Only benzylguaninetagged plasma membrane proteins that are transported via the retrograde route can covalently couple to a TGNlocalized SNAPtag fusion protein. The approach was validated stepbystep using a well explored retrograde cargo protein, the Bsubunit of Shiga toxin. It was then extended in an unbiased manner to the proteomics format. This allowed identifying an endogenous protein that was one of the first retrograde cargo proteins to be described in the past. In conclusion, we have pioneered a dynamic proteomics approach that complements traditional approaches in the study of protein trafficking. Moreover, the concept that is described here is of generic nature and may be extended to other endocytic pathways.

⚠️ WARNING (California Proposition 65):

This product may contain chemicals known to the State of California to cause cancer, birth defects, or other reproductive harm.

For more information, please visit www.P65Warnings.ca.gov.

Recently Viewed