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Pyrosequencing Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing technology, all written by worldrenowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing, and supplementing each protocol with troubleshooting tips specific to that method.Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing technique, and Chapters 57 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing comprise Chapters 813, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing.
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