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RNA molecules participate in and regulate a vast array of cellular processes, and the scientific community is now entering a new era in which some aspect of RNA biologyas a tool, a therapeutic, a diagnostic, or part of a fundamental processis becoming increasingly important. But initiating RNA research can be intimidating, and without a thorough understanding of the challenges and complexities inherent in handling this fragile nucleic acid, forays into the RNA world can be quite frustrating. RNA: A Laboratory Manual provides a broad range of uptodate techniques so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the most sophisticated. Originating in four of the field's most prominent laboratories and written with novices as well as more advanced researchers in mind, this manual provides the necessary background and strategies for approaching any RNA investigation in addition to detailed stepbystep protocols and extensive tips and troubleshooting information. RNA: A Laboratory Manual will enable any researcher to approach a wide variety of RNArelated problems with confidence and a high expectation of success.
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