Title
RNA Interference in insect cells infected with baculovirus: A universal transgene silencing approach,Used
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Baculovirus?insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi) with BICS. In this work, the enhanced green fluorescent protein (EGFP) was used as the RNAi consistent target sequence located downstream of a depressant insectneurotoxin gene, LqqIT2 used as a model of the gene of interest. Small interfering RNA (siRNA) and inverted repeats of EGFP gene (IREG) were examined in targeting the EGFPLqqIT2 (EL)fusion mRNA or LqqIT2EGFP (LE)bicistronic mRNA for degradation. Suppression efficiencies using these inducers were examined transiently and stably in uninfected and infected insect Sf9 cells.
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