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STED microscopy as a tool in cell biology: Investigation of agerelated alterations occurring on the protein and the lipid level,Used
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This thesis reports on two applications of the STED (stimulated emission depletion) principle investigating alterations on the protein and lipid level. One hallmark of agerelated neurodegenerative diseases is the abnormal posttranslationally hyperphosphorylation of proteins. Here, the interplay of two main posttranslational modifications of neurofilaments was investigated. The organization pattern of these modifications that were not resolvable by conventional light microscopy could finally be displayed with the about 10times higher resolution of STED microscopy. Furthermore this technique allowed the detection of early alterations in these patterns in dependence of the provided glucose amount. Another effect occurring during aging is the change in the plasma membrane''s fluidity. This thesis reports on the investigation of the diffusion characteristics of different phospholipids using STEDFCS in living cells. It was shown that sphingolipids, in contrast to phosphoglycerolipids, were trapped on spatial scales below 30 nm, which could not be examined by confocal microscopy. Upon changing the endogenous cholesterol level this hindered diffusion could be reversibly abolished.
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