Stem Cell Transcriptional Networks: Methods and Protocols (Methods in Molecular Biology, 2117),Used

Stem Cell Transcriptional Networks: Methods and Protocols (Methods in Molecular Biology, 2117),Used

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This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote selfrenewal and differentiation. Chapters detail nextgeneration sequencing technologies, data analysis, protocols on analysis and visualization of singlecell RNASeq data, analysis of 3D chromatin architecture, interpretation of largescale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cuttingedge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.

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