Strategies for Protein Purification and Characterization: A Laboratory Course Manual,New

Strategies for Protein Purification and Characterization: A Laboratory Course Manual,New

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Investigators who have identified and cloned a gene of interest often want to isolate and characterize the protein product, yet the methods required are notoriously tricky for the inexperienced. For the past four years, a course has been held at Cold Spring Harbor Laboratory to teach scientists how to execute the major protein techniques by applying them to four distinct, representative types of molecule: a regulatory protein, a DNAbinding protein, a recombinant protein, and a membranebound receptor. This course has now been adapted in the form of a laboratory manual that covers a variety of bulk fractionation, electrophoretic, and chromatographic techniques. Stepbystep protocols are accompanied by troubleshooting advice and guidance on generalizing the techniques for other classes and types of protein. The emphasis throughout is on strategies for purification and characterization rather than automated instrumental analysis. After years of rigorous testing, these techniques are robust and reliable, and are presented here with the clarity and completeness for which Cold Spring Harbor manuals are celebrated. The book is invaluable for specialists in genetics, microbiology, neuroscience, and cell biology who wish to develop expertise in working with proteins.

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To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.

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