Title
Studies on divergent CRPdependent promoters,Used
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Bacterial genomes contain genes that are packed closely together. For many genes, promoter regions overlap, and regulatory segments that carry divergent promoters are common. In this study, I have focussed on promoters that are regulated by the cyclic AMP receptor protein (CRP) of Escherichia coli. CRP is a global regulator that directs the activity of more than 100 genes, some of which are oriented backtoback, i.e., in a divergent fashion. CRP is known to activate transcription initiation through direct proteinprotein contact with RNA polymerase and CRPdependent promoters are classified into two classes, Class I and Class II, according to the location of the CRPbinding site. I have investigated whether a single bound CRP, either at a Class I or a Class II location, can activate divergent transcription.
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