Title
Studies on the Citrus Tristeza Virus and Citrus Viroids in Pakistan: Viral and Viroids Diseases of Citrus,Used
Delivery time: 8-12 business days (International)
Citrus tristeza virus (CTV) is distributed worldwide, and is the causal agent of one of the most economically important and destructive diseases of citrus.Direct tissue blot immunoassay (DTBIA) and reverse transcription polymerase chain reaction (RTPCR) were used to preselect CTV isolates. CTVinfected samples were then preserved in the sweet orange by graftinoculation. The target sequences of the major coat protection gene (p25 gene) were amplified from the CTVinfected samples using RTPCR, and the p25 genes of these isolates were analyzed by RFLP and SSCP for investigating the p25/Hinf I RFLP groups. Genetic differences and genetic relationships and the sequences of 4 genomic regions p23, p20, p18, and p25 of the 21 CTV isolates were amplified and sequenced using RTPCR and with a sequencer. Using sequence analysis, the differences were therefore estimated among the CTV isolates from cultivated citrus. Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd), Hop stunt viroid (HSVd), Citrus dwarfing viroid (CDVd), and Citrus bark cracking viroid (CBCVd) were detected by onestep multiplex RTPCR assay simultaneously.
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Warranty
We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
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