Title
Techniques for the Analysis of Complex Genomes,Used
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From the Back Cover The search for the genes associated with human diseases has initiated the need for new strategies to map and clone large regions of DNA with the prospect of mapping and sequencing the human genome and those of other organisms. Containing contributions from some of the leading laboratories in the world, Techniques for the Analysis of Complex Genomes describes genomic mapping by pulsed field gel electrophoresis and provides a detailed account of the cloning of large fragments of DNA using yeast artificial chromosomes (YACs) and bacteriophage PI cloning systems.@introbul:Spirally bound for easy use at the laboratory bench, this volume describes:@bul:* Use of methylationsensitive enzymes for mapping large genomic regions by pulsed field gel electrophoresis* Stepbystep protocols for the construction, characterisation, screening and use of PI libraries* Stepbystep protocols for the construction, characterisation, screening and use of YAC libraries* The analysis of YAC's, their use in chromosome walking, and how to detect and deal with their instability* Examples on the successful use of YACs for cloning human telomeres, screening cDNA libraries and reconstruction of large genes like that of the Duchenne Muscular Dystrophy gene* Homologous recombination permitting the insertion of selectable markers into YACs prior to their transfer into mammalian cells* Automated laboratory procedures for screening libraries and their potential use in generating hybridisation fingertips for genome mapping* The use of microdissection to generate large number of probes from defined chromosomal regions* Extensive referencing to recently published primary literature* Cross referencing between chapters Product Description Faced with the search for genes associated with human diseases and the prospect of mapping and sequencing the human genome and those of other organisms, scientists must employ new strategies designed to map and clone large regions of DNA. Written by internationally renowned scientists, and containing contributions from some of the leading laboratories in the world, Techniques for the Analysis of Complex Genomes describes genomic mapping by pulsed field gel electrophoresis, and provides a detailed account of the cloning of large fragments of DNA using yeast artificial chromosomes (YACs) and bacteriophage PI cloning systems.
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We ship your order within 2–3 business days for USA deliveries and 5–8 business days for international shipments. Once your package has been dispatched from our warehouse, you'll receive an email confirmation with a tracking number, allowing you to track the status of your delivery.
Returns
To facilitate a smooth return process, a Return Authorization (RA) Number is required for all returns. Returns without a valid RA number will be declined and may incur additional fees. You can request an RA number within 15 days of the original delivery date. For more details, please refer to our Return & Refund Policy page.
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Warranty
We provide a 2-year limited warranty, from the date of purchase for all our products.
If you believe you have received a defective product, or are experiencing any problems with your product, please contact us.
This warranty strictly does not cover damages that arose from negligence, misuse, wear and tear, or not in accordance with product instructions (dropping the product, etc.).
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