Title
Techniques In Free Radical Research (Volume 22) (Laboratory Techniques In Biochemistry And Molecular Biology, Volume 22)
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Free radical species are generally shortlived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spintrapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or 'FOOTPRINTS' of radicalmediated damage.Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radicalmediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.
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