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The Hypertrophied Heart: Biophysical, biochemical, and morphological aspects of hypertrophy. International Erwin Riesch Symposiu,Used
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Die Umkehr der CalciumPumpe des myokardialen sarkoplasmatischen Retikulums (SR) wurde untersucht. SRVesikel, die passiv durch Prliinkubation mit hohen Calciumkonzentrationen mit Calcium beladen wurden, synthetisieren ATP aus Orthophosphat und ADP. Die ATPSynthese erfolgt tiber die Phos phorylierung der CalciumTransportATPase des SR durch Orthophosphat und eine Dephosphorylierung des E '""" P durch ADP. Die Rate der CalciumFrei setzung aus SRVesikel die CalciumBeladung erfolgte aktiv in Gegenwart von Acetylphosphat wird durch ADP erhoht. Der ADPabhlingige Calcium Efflux ist stochiometrisch mit der ATPSynthese aus Orthophosphat und ADP gekoppelt. References 1. Suko, J.: The calcium pump of cardiac sarcoplasmic reticulum. Functional alterations at different levels of thyroid state in rabbits. J. Physiol. 228, 563 (1973). 2. Hasselbach, W.: Sarcoplasmic Membrane. ATPase. In: The Enzymes (P. D. Boyer, eds.), N. Y. 10, 431 (1974). 3. Pang, D. C., N. F. Briggs: Reaction mechanism of cardiac sarcotuble calcium dependent adenosin triphosphatase. Biochemistry 12, 4905 (1973). 4. Suko, J., W. Hasselbach: Properties of a phosphorylated intermediate of the 2 Ca dependent ATPase and ADPATP phosphate exchange of cardiac sarco plasmic reticulum. NaunynSchmiedeberg's Arch. Pharmakol. expo Pathol. 282, R. 97 (1974). 5. Suko, J., W. Hasselbach: Characterization of cardiac sarcoplasmic reticulum ATPADP phosphate exchange and phosphorylation of the calcium trans port adenosin triphosphatase. Europ. J. Biochem. 64, 123 (1976). 2 2 6. Suko, J.: The effect of temperature on Ca ]uptake and Ca +activated ATP hydrolysis by cardiac sarcoplasmic reticulum. Experientia 29, 396 (1973).
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