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Transcription Regulation In Prokaryotes,New
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Transcription is part of the process of gene expression and is very different in prokaryotes and eukaryotes. In prokaryotic cells the synthesis reactions of DNA, RNA and proteins are intricately coupled as there are no subcellular compartments. Thus replication, transcription and translation occur simultaneously without local separation and the regulations of each is interrelated. In bacteria, regulation is most important at the transcription stage of the biosynthetic process, and changes in the transcriptional efficiency account for major differences in the frequency of RNA formation and gene expression. This textbook has been written by an authority in the field for advanced students and postgraduates. Both clear and concise, there is an emphasis on transcription in bacterial and phage expression systems, a key area of study and research in molecular biology. Many general mechanisms and principles emerge from studies of prokaryotic transcription complexes that help build an understanding of regulation in higher organisms.Taking a biophysical approach, the text draws together elements of molecular biology, genetics, structural biology and biochemistry to provide an invaluable summary of the field of prokaryotic transcription regulation for students in molecular biology and related areas of study such as microbiology and biotechnology.Additional content information: this book includesnew data on the composition and architecture of RNA polmerase new findings on sigma and antisigma factorsa description of promoter elements underlining the importance of upstream and downstream flanking sequencesdetailed kinetic description of the initiation processnew data on the function of DNA curvature and topology on transcriptiongeneral mechanisms of repressionpositive regulationtermination, antitermination and attenuation mechanismsimplications of the nascent RNA structure in regulationstringent control mechanisms and global networkscomparative chapter for parallel and divergent mechanisms in prokaryotic and eukaryotic transcription regulationmodern methods of analysis and new technical developments with selected examples
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